Choquet C G, Patel G B, Beveridge T J, Sprott G D
Institute for Biological Sciences, National Research Council of Canada (NRCC), Ottawa, Ontario.
Appl Microbiol Biotechnol. 1994 Nov;42(2-3):375-84. doi: 10.1007/BF00902745.
Ether lipids were obtained from a wide range of archaeobacteria grown at extremes of pH, temperature, and salt concentration. With the exception of Sulfolobus acidocaldarius, unilamellar and/or multilamellar liposomes could be prepared from emulsions of total polar lipid extracts by pressure extrusion through filters of various pore sizes. Dynamic light scattering, and electron microscopy revealed homogeneous liposome populations with sizes varying from 40 to 230 nm, depending on both the lipid source and the pore size of the filters. Leakage rates of entrapped fluorescent or radioactive compounds established that those archaeobacterial liposomes that contained tetraether lipids were the most stable to high temperatures, alkaline pH, and serum proteins. Most ether liposomes were stable to phospholipase A2, phospholipase B and pancreatic lipase. These properties of archaeobacterial liposomes make them attractive for applications in biotechnology.
醚脂是从生长在极端酸碱度、温度和盐浓度条件下的多种古细菌中获取的。除了嗜酸热硫化叶菌外，通过不同孔径的滤膜进行压力挤压，可以从总极性脂质提取物的乳液中制备出单层和/或多层脂质体。动态光散射和电子显微镜显示，脂质体群体均匀，其大小从40纳米到230纳米不等，这取决于脂质来源和滤膜的孔径。包封的荧光或放射性化合物的泄漏率表明，那些含有四醚脂质的古细菌脂质体对高温、碱性pH值和血清蛋白最稳定。大多数醚脂质体对磷脂酶A2、磷脂酶B和胰脂肪酶都很稳定。古细菌脂质体的这些特性使其在生物技术应用中具有吸引力。
