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[在微量元素研究中使用床边卡片作为PCR技术二次对照的可能性]

[Possibilities for using bedside cards as secondary comparison in trace element studies with the PCR technique].

作者信息

Du Chesne A, Schütte U, Brinkmann B

机构信息

Institut für Rechtsmedizin, Westfälischen Wilhelms-Universität Münster.

出版信息

Arch Kriminol. 1992 Sep-Oct;190(3-4):103-8.

PMID:1444705
Abstract

When fresh blood is not available as a control in stain investigations extracted teeth, hair, preserved tissue samples, histological slides, cigarette butts or used stamps can also be used. This paper reports on a stain investigation performed 7 months after the death of the victim, where a bedside card from the medical records was successfully employed as a control blood sample. In a series of 10 bedside cards up to 9 years old, the investigation with the PCR method showed recognizable patterns in the STR systems SE 33 and TC 11. Matching patterns could be found from the 4 sections of each card (anti A, anti B, anti AB and anti D). A comparison of the oldest card with a fresh blood sample of the patient also showed matching patterns. AMPFLPs were successful with more recent cards. Using the PCR method typing of bedside cards from medical records up to 10 years old can be used in stain investigations.

摘要

在血迹调查中,若无法获取新鲜血液作为对照,也可使用拔除的牙齿、毛发、保存的组织样本、组织学切片、烟头或用过的邮票。本文报道了在受害者死亡7个月后进行的一次血迹调查,其中病历中的床头卡被成功用作对照血样。在一系列多达9年历史的10张床头卡中,采用聚合酶链反应(PCR)方法进行的调查显示,在短串联重复序列(STR)系统SE 33和TC 11中呈现出可识别的图谱。每张卡片的4个部分(抗A、抗B、抗AB和抗D)都能找到匹配的图谱。将最旧的卡片与患者的新鲜血样进行比较,也显示出匹配的图谱。扩增片段长度多态性(AMPFLP)方法在更新的卡片上取得了成功。使用PCR方法对长达10年历史的病历床头卡进行分型,可用于血迹调查。

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