Beezhold D, Beck W C
Laboratory of Macrophage Biology, Guthrie Foundation for Medical Research, Sayre, Pa. 18840.
Arch Surg. 1992 Nov;127(11):1354-7. doi: 10.1001/archsurg.1992.01420110102020.
Latex surgical gloves have recently been identified as a potential source of allergens. Much of the current information suggests that the soluble proteins in latex may cause significant reactions in sensitive individuals. The starch powders used as a lubricant on some latex gloves have also been identified as potential allergens in some patients. In this study, we determined these powders to act as potential carriers of latex allergens. We have produced a polyclonal antiserum to be used as a reagent to study latex proteins. By Western blot analysis, we identified a significant interaction between latex proteins and starch powders. The binding of latex proteins to starch particles results in a glove particle that may have an increased potential to act as an allergen. The latex protein-starch particles represent a potential mechanism for exposure and sensitization of health care workers to latex allergens. Elimination of these particles from the operating room should reduce the route of sensitization and the potential for adverse reactions to latex.
乳胶手术手套最近被确定为一种潜在的过敏原来源。目前的许多信息表明，乳胶中的可溶性蛋白质可能会在敏感个体中引起严重反应。一些乳胶手套上用作润滑剂的淀粉粉末在一些患者中也被确定为潜在的过敏原。在本研究中，我们确定这些粉末可作为乳胶过敏原的潜在载体。我们制备了一种多克隆抗血清用作研究乳胶蛋白的试剂。通过蛋白质免疫印迹分析，我们确定乳胶蛋白与淀粉粉末之间存在显著相互作用。乳胶蛋白与淀粉颗粒的结合产生了一种手套颗粒，其作为过敏原的可能性可能会增加。乳胶蛋白 - 淀粉颗粒代表了医护人员接触和致敏乳胶过敏原的一种潜在机制。从手术室消除这些颗粒应可减少致敏途径以及对乳胶产生不良反应的可能性。
