Beezhold D H, Sussman G L, Kostyal D A, Chang N S
Guthrie Research Institute, Sayre, PA 18840.
Clin Exp Immunol. 1994 Dec;98(3):408-13. doi: 10.1111/j.1365-2249.1994.tb05505.x.
Latex allergy is an occupational hazard for health care workers. Extractable latex proteins are known to be allergenic, but most latex allergens have not been specifically identified. The purpose of this study was to characterize the IgE response of latex-allergic patients to latex proteins and to identify common protein allergens. Serum was obtained from 40 individuals who were skin test-positive to latex; 85% were health care workers. Western blots for IgE reactivity were performed using both ammoniated (AL) and non-ammoniated (NAL) latex proteins and IgE-reactive NAL proteins were analysed by microsequence analysis. The patients were grouped according to common patterns of reactivity. Pattern 1, the most common pattern of reactivity (9/40 patients) recognized two protein bands in both NAL and AL at 46 and 110 kD. A second, heterogeneous pattern of reactivity (pattern 2) recognized a diffuse pattern of polypeptides in the AL preparation. The n-terminal amino acid sequences for allergens at 14, 18, 29, 46 and 110 kD were determined. Sequence analysis identified the 14-kD and 18-kD allergens as the hevein proprotein. The 46-kD and 110-kD had identical sequences which were unique from known latex proteins. We conclude that multiple latex proteins are allergens with hevein preprotein and a previously unidentified 46/110-kD protein being commonly recognized in health care workers.
乳胶过敏是医护人员面临的职业危害。已知可提取的乳胶蛋白具有致敏性，但大多数乳胶过敏原尚未得到明确鉴定。本研究的目的是描述乳胶过敏患者对乳胶蛋白的IgE反应，并鉴定常见的蛋白质过敏原。从40名乳胶皮肤试验呈阳性的个体中获取血清；其中85%为医护人员。使用氨化（AL）和非氨化（NAL）乳胶蛋白进行IgE反应性的蛋白质印迹分析，并通过微序列分析对IgE反应性NAL蛋白进行分析。根据常见的反应模式对患者进行分组。模式1是最常见的反应模式（40名患者中的9名），在NAL和AL中均识别出46kD和110kD的两条蛋白带。第二种异质性反应模式（模式2）在AL制剂中识别出弥散的多肽模式。测定了14kD、18kD、29kD、46kD和110kD过敏原的N端氨基酸序列。序列分析确定14kD和18kD过敏原为橡胶素前体蛋白。46kD和110kD具有相同的序列，这与已知的乳胶蛋白不同。我们得出结论，多种乳胶蛋白是过敏原，橡胶素前体蛋白和一种先前未鉴定的46/110kD蛋白在医护人员中普遍被识别。
