Rao T K, DeBusk A G
Biochim Biophys Acta. 1977 Nov 1;470(3):475-83. doi: 10.1016/0005-2736(77)90138-9.
Neurospora crassa conidia possess an active transport system for the uptake of acetate. This system was characterized as: (a) energy dependent; (b) taking place against a concentration gradient; (c) saturating at higher substrate concentrations and (d) competitively inhibited by propionate. Activity of the acetate transport system can be further enhanced by preincubating conidia in 1 mM acetate medium for 180 min (the inducible transport system). The conidial system and the inducible system have similar properties. The development of the inducible transport was dependent on RNA and protein synthesis. A genetic control of this system was further confirmed by isolating a mutant acp-i acetate permease, inducible) that fails to develop the inducible transport system.
粗糙脉孢菌分生孢子拥有一个用于摄取乙酸盐的主动运输系统。该系统的特征如下：(a) 依赖能量；(b) 逆浓度梯度进行；(c) 在较高底物浓度下达到饱和；以及 (d) 被丙酸盐竞争性抑制。通过将分生孢子在1 mM乙酸盐培养基中预孵育180分钟（诱导型运输系统），乙酸盐运输系统的活性可进一步增强。分生孢子系统和诱导型系统具有相似的特性。诱导型运输的发展依赖于RNA和蛋白质合成。通过分离出一个突变体acp-i（乙酸盐通透酶，可诱导型），该突变体无法形成诱导型运输系统，进一步证实了该系统的遗传控制。
