Sharma R, Dey S, Verma R
Department of Biochemistry, North-Eastern Hill University, Shillong, India.
Biochem Int. 1992 Sep;27(6):1059-66.
The activities of malate-aspartate shuttle enzymes were measured in the liver and kidney of 15-, 30-, and 60-day old mice. The results indicate that the activities (U/mg protein) of both isoenzymes (cytosolic and mitochondrial) of both malate dehydrogenase and aspartate aminotransferase are significantly higher in the liver of 15-day old mice than in the liver of 30- and 60-day old animals. However, the shuttle enzymes showed a peak value in the kidney of 30-day old mice. In vitro reconstitution of malate-aspartate shuttle showed a similar pattern of activity in the tissues studied. These findings suggest that the activity of malate-aspartate shuttle is expressed differentially in these tissues of mice at different postnatal ages.
在15日龄、30日龄和60日龄小鼠的肝脏和肾脏中测定了苹果酸-天冬氨酸穿梭酶的活性。结果表明，15日龄小鼠肝脏中苹果酸脱氢酶和天冬氨酸转氨酶的两种同工酶（胞质和线粒体）的活性（U/mg蛋白）均显著高于30日龄和60日龄动物的肝脏。然而，穿梭酶在30日龄小鼠的肾脏中显示出峰值。苹果酸-天冬氨酸穿梭的体外重组在研究的组织中显示出类似的活性模式。这些发现表明，苹果酸-天冬氨酸穿梭的活性在出生后不同年龄的小鼠这些组织中差异表达。
