KORNER N H
J Clin Pathol. 1962 May;15(3):195-9. doi: 10.1136/jcp.15.3.195.
A technique for measuring the alkaline phosphatase activity of serum protein fractions separated by electrophoresis on cellulose acetate strips is described. Alkaline phosphatase activity in alpha 2, alpha 1, and beta globulins and in albumin is present in varying proportions in normal and pathological sera. In normal sera peak activity is in the alpha 2 globulins and beta globulins have a little more than half the alpha 2 activity. Albumin activity is often more than 10% of the total, but alpha 1 and gamma globulin activity is less than this. In bone disorders with a high serum alkaline phosphatase a large, and sometimes predominant, part of the increase is due to raised beta globulin phosphatase activity, and alpha 2 activity is also raised. Alpha 1 activity, though usually low, may be increased. In hepatobiliary disorders with a high phosphatase concentration the increase is due mainly to raised alpha 2 phosphatase activity, and there is also an increase in alpha 1 phosphatase. Beta activity is usually, but not always, low, and albumin activity may be somewhat increased in obstructive jaundice and liver metastases. There is some overlap between these two patterns of alkaline phosphatase distribution. The findings are consistent with the possibility that alkaline phosphatases in different protein fractions are derived from different tissues.
本文描述了一种测量在醋酸纤维素条上通过电泳分离的血清蛋白组分碱性磷酸酶活性的技术。正常和病理血清中，α2、α1和β球蛋白以及白蛋白中的碱性磷酸酶活性比例各不相同。正常血清中，峰值活性存在于α2球蛋白中，β球蛋白的活性略高于α2活性的一半。白蛋白活性通常超过总量的10%，但α1和γ球蛋白活性低于此值。在血清碱性磷酸酶升高的骨骼疾病中，升高的很大一部分，有时甚至是主要部分，是由于β球蛋白磷酸酶活性升高所致，α2活性也会升高。α1活性虽然通常较低，但可能会增加。在磷酸酶浓度升高的肝胆疾病中，升高主要是由于α2磷酸酶活性升高，α1磷酸酶也会增加。β活性通常较低，但并非总是如此，在梗阻性黄疸和肝转移中白蛋白活性可能会有所增加。这两种碱性磷酸酶分布模式之间存在一些重叠。这些发现与不同蛋白质组分中的碱性磷酸酶可能来自不同组织的可能性一致。
