Chiasson B J, Hooper M L, Murphy P R, Robertson H A
Department of Pharmacology, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada.
Eur J Pharmacol. 1992 Dec 1;227(4):451-3. doi: 10.1016/0922-4106(92)90167-t.
Immediate-early genes such as c-fos and NGFI-A are rapidly and transiently expressed in the striatum following amphetamine administration in vivo. Here we show that direct infusion of an antisense oligodeoxynucleotide to c-fos into striatum will reduce amphetamine-induced production of Fos-like immunoreactivity without affecting NGFI-A expression. These results suggest that it is possible to use antisense technology to study the role of immediate-early genes in specific sites in the brain in vivo.
诸如c-fos和NGFI-A等即早基因在体内给予苯丙胺后会在纹状体中快速且短暂地表达。在此我们表明，将针对c-fos的反义寡脱氧核苷酸直接注入纹状体，会减少苯丙胺诱导的Fos样免疫反应性的产生，而不影响NGFI-A的表达。这些结果表明，利用反义技术在体内研究即早基因在脑内特定部位的作用是可能的。
