MARCINIAK E, RODRIGUEZ-ERDMANN F, SEEGERS W H
Science. 1962 Aug 10;137(3528):421-2. doi: 10.1126/science.137.3528.421.
Pure bovine thrombin was separated from autoprothrombin C, and the lethal intravenous dose for mice weighing 25 g was 0.4 ml of a solution containing 50 units of thrombin per milliliter. Autoprothrombin C was not toxic alone, but with crude cephalin it was fatal. The clotting time of human plasma was only slightly accelerated by autoprothrombin C alone; the clotting time was as short as 5 seconds with a combination of autoprothrombin C and cephalin.
纯牛凝血酶从自身凝血酶原C中分离出来，对于体重25克的小鼠，静脉注射致死剂量是每毫升含50单位凝血酶的溶液0.4毫升。自身凝血酶原C单独使用无毒，但与粗脑磷脂一起使用则致命。单独使用自身凝血酶原C时，人血浆的凝血时间仅略有加快；自身凝血酶原C与脑磷脂联合使用时，凝血时间可短至5秒。
