Cuffini A M, Tullio V, Fazari S, Allocco A, Carlone N A
Institute of Microbiology, University of Turin, Italy.
Int J Tissue React. 1992;14(3):131-9.
Antibiotic potentiation of host defence mechanisms may be of potential clinical importance in the outcome of infections. Therefore the effect of pefloxacin upon the interaction of in vitro of human macrophages with Klebsiella pneumoniae, by assays of antibiotic cellular uptake, bacterial phagocytosis and intracellular killing, was examined. The results indicated that pefloxacin was well concentrated by phagocytes at all the concentrations tested. The uptake proceeded rapidly and was not affected either by cell viability or physiological environmental temperature. Synergistic phagocytosis and intracellular killing of K. pneumoniae was observed in the presence of macrophages and subinhibitory concentrations (one-half MIC) of pefloxacin. Pretreatment of bacteria with pefloxacin led to an increase in both bacterial uptake and microbicidal activity of phagocytes. Exposure of the macrophages to pefloxacin did not affect any phagocyte functions.
抗生素对宿主防御机制的增强作用在感染的转归中可能具有潜在的临床重要性。因此，通过抗生素细胞摄取、细菌吞噬和细胞内杀伤试验，研究了培氟沙星对人巨噬细胞与肺炎克雷伯菌体外相互作用的影响。结果表明，在所有测试浓度下，培氟沙星都能被吞噬细胞很好地摄取。摄取过程迅速，不受细胞活力或生理环境温度的影响。在存在巨噬细胞和亚抑菌浓度（半数MIC）的培氟沙星时，观察到对肺炎克雷伯菌的协同吞噬作用和细胞内杀伤作用。用培氟沙星预处理细菌导致吞噬细胞对细菌的摄取和杀菌活性均增加。巨噬细胞暴露于培氟沙星并不影响任何吞噬细胞功能。
