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从R质粒NR1的拷贝数突变体产生微型质粒。

Generation of miniplasmids from copy number mutants of the R plasmid NR1.

作者信息

Taylor D P, Greenberg J, Rownd R H

出版信息

J Bacteriol. 1977 Dec;132(3):986-95. doi: 10.1128/jb.132.3.986-995.1977.

DOI:10.1128/jb.132.3.986-995.1977
PMID:144723
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC235601/
Abstract

Small, closed circular deoxyribonucleic acid molecules, called miniplasmids, were observed in Escherichia coli harboring copy number mutants of the R plasmid NR1 after growth in medium containing tetracycline. The level of tetracycline resistance conferred by the copy mutant plasmids was lower (3 to 6 microgram/ml) than that conferred by NR1 (100 MICROGRAM/ML). The presence of the miniplasmid enhanced the level of tetracycline resistance conferred by the copy mutant. Miniplasmids of molecular weights 4 X 10(6) to 13 X 10(6) were found. They carried no antibiotic resistance markers and could be eliminated by growth in the presence of chloramphenicol and/or streptomycin-spectinomycin. Studies with the restriction endonucleases EcoRI and Sal I indicated that the miniplasmids are derived from the region of the copy mutant plasmids that contains the origin for replication of the resistance transfer factor. There were approximately 12 copies of the miniplasmid per chromosome, compared with 3 and 6 copies of the copy mutants of NR1. The miniplasmids appeared to be incompatible with the copy mutant plasmids.

摘要

在含有四环素的培养基中生长后，在携带R质粒NR1拷贝数突变体的大肠杆菌中观察到了称为微型质粒的小型闭合环状脱氧核糖核酸分子。拷贝突变体质粒赋予的四环素抗性水平（3至6微克/毫升）低于NR1赋予的水平（100微克/毫升）。微型质粒的存在提高了拷贝突变体赋予的四环素抗性水平。发现了分子量为4×10⁶至13×10⁶的微型质粒。它们不携带抗生素抗性标记，并且可以通过在氯霉素和/或链霉素-壮观霉素存在下生长而消除。用限制性内切酶EcoRI和Sal I进行的研究表明，微型质粒源自拷贝突变体质粒中包含抗性转移因子复制起点的区域。每条染色体上大约有12个微型质粒拷贝，而NR1拷贝突变体分别有3个和6个拷贝。微型质粒似乎与拷贝突变体质粒不相容。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d3d/235601/78d46e5bc0ea/jbacter00301-0256-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d3d/235601/6912292aac53/jbacter00301-0254-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d3d/235601/d6129131c292/jbacter00301-0255-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d3d/235601/78d46e5bc0ea/jbacter00301-0256-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d3d/235601/6912292aac53/jbacter00301-0254-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d3d/235601/d6129131c292/jbacter00301-0255-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d3d/235601/78d46e5bc0ea/jbacter00301-0256-a.jpg

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引用本文的文献

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J Bacteriol. 1993 Jul;175(14):4554-60. doi: 10.1128/jb.175.14.4554-4560.1993.
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Regular segregation of composite plasmid Rms201.复合质粒Rms201的常规分离
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Biochemical and immunological characterization of an R plasmid-encoded protein with properties resembling those of major cellular outer membrane proteins.

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Mutations in R factors of Escherichia coli causing an increased number of R-factor copies per chromosome.大肠杆菌R因子中的突变导致每条染色体上R因子拷贝数增加。
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