使用气相色谱法和火焰离子化检测法测定血清中的甲基丙烯腈。

Determination of methacrylonitrile in serum using gas chromatography and flame ionization detection.

作者信息

Demby K B, White T C, Ghanayem B I

机构信息

National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.

出版信息

J Chromatogr. 1992 Aug 7;579(1):153-7. doi: 10.1016/0378-4347(92)80373-x.

DOI:10.1016/0378-4347(92)80373-x

PMID:1447342

Abstract

A gas chromatographic method is described for the quantitation of methacrylonitrile in serum. Methacrylonitrile was extracted from rat serum with diethyl ether and then quantified using a gas chromatograph equipped with a flame ionization detector and a 60-m megabore column coated with polyethylene glycol polymer. The recoveries obtained following a one-step extraction with diethyl ether varied from 60% at 3.2 micrograms/ml to 70% at 80 micrograms/ml. The coefficient of variation for the analysis ranged from 2.5% at 400 micrograms/ml to 15.0% at 3.2 micrograms/ml.

摘要

描述了一种用于定量血清中甲基丙烯腈的气相色谱法。用乙醚从大鼠血清中提取甲基丙烯腈，然后使用配备火焰离子化检测器和涂有聚乙二醇聚合物的60米大口径柱的气相色谱仪进行定量。用乙醚一步提取后获得的回收率在3.2微克/毫升时为60%，在80微克/毫升时为70%。分析的变异系数在400微克/毫升时为2.5%，在3.2微克/毫升时为15.0%。