MIDGLEY A R, PIERCE G B
J Exp Med. 1962 Feb 1;115(2):289-94. doi: 10.1084/jem.115.2.289.
Through the use of immunohistochemical techniques, human chorionic gonadotropin has been localized to syncytiotrophoblastic cells of immature placenta, hydatidiform mole, chorioadenoma destruens, and choriocarcinoma. No gonadotropin has been detected in cytotrophoblast. Evidence is discussed which suggests that syncytiotrophoblast is the cell of origin of human chorionic gonadotropin. The observation that formalin fixation did not alter the ability of human chorionic gonadotropin to react with its specific antibody permitted the study of formalin-fixed paraffin-embedded tissues stored in the tissue collection. In addition, the excellence of histologic preparations following formalin fixation facilitated cytologic identification.
通过免疫组织化学技术,已将人绒毛膜促性腺激素定位于未成熟胎盘、葡萄胎、破坏性绒毛膜腺瘤和绒毛膜癌的合体滋养层细胞中。在细胞滋养层中未检测到促性腺激素。文中讨论了相关证据,这些证据表明合体滋养层是人绒毛膜促性腺激素的起源细胞。福尔马林固定并未改变人绒毛膜促性腺激素与其特异性抗体反应的能力,这一观察结果使得对保存在组织库中的福尔马林固定石蜡包埋组织进行研究成为可能。此外,福尔马林固定后组织学切片的优良质量有助于细胞学鉴定。