MILLINGTON P F, FINEAN J B
J Cell Biol. 1962 Jul;14(1):125-39. doi: 10.1083/jcb.14.1.125.
Immersion of the intestinal tissue, from rat jejunum, in hypertonic saline produced very rapid changes in all regions of the epithelial cells, but the apical region was apparently unaffected by hypotonic solutions for at least (1/2) hour. In both cases, blistering of the microvilli was taken as the first sign of degenerative changes which finally resulted in a breakdown to large vesicular particles. Consideration of both normal and modified tissue indicates that the core of the microvillus contains either paired strands or tubular structures. Lateral cross-fibres extended from the core to the microvillus membrane and may be an essential part of the supporting structure of the microvillus. Densitometer traces across the microvillus membrane at various stages of modification indicated that this membrane might include a 75 A unit membrane structure with additional components associated at either surface. Interruptions in the membrane were apparently expanded by the hypotonic solutions and these might possibly be distinguished from preparative artefacts.
将大鼠空肠的肠道组织浸入高渗盐水中会使上皮细胞的所有区域迅速发生变化，但顶端区域在至少半小时内显然不受低渗溶液的影响。在这两种情况下，微绒毛起泡被视为退行性变化的首个迹象，最终导致分解为大的囊泡颗粒。对正常组织和经处理组织的研究表明，微绒毛的核心包含成对的股线或管状结构。横向交叉纤维从核心延伸至微绒毛膜，可能是微绒毛支撑结构的重要组成部分。在不同处理阶段对微绒毛膜进行的密度计扫描显示，该膜可能包括一个75埃的单位膜结构，其表面还附着有其他成分。膜中的中断处显然因低渗溶液而扩大，这些中断可能与制备过程中的假象有所不同。
