Yildiz F H, Gest H, Bauer C E
Department of Biology, Indiana University, Bloomington 47405.
Mol Microbiol. 1992 Sep;6(18):2683-91. doi: 10.1111/j.1365-2958.1992.tb01445.x.
Intraspecies and intergenus complementation analysis were utilized to demonstrate that photosynthesis genes are clustered in distantly related purple photosynthetic bacteria. Specifically, we show that the linkage order for genes involved in bacteriochlorophyll and carotenoid biosynthesis in Rhodospirillum centenum are arranged essentially as in Rhodobacter capsulatus and Rhodobacter sphaeroides. In addition, the location and relative distance observed between the puf and puh operons which encode for light harvesting and reaction-centre structural genes are also conserved between these species. Conservation of the photosynthesis gene cluster implies either that there are structural or regulatory constraints that limit rearrangement of the photosynthesis gene cluster or that there may have been lateral transfer of the photosynthesis gene cluster among different species of phototrophic bacteria.
利用种内和属间互补分析来证明光合作用基因在远缘相关的紫色光合细菌中是成簇的。具体而言，我们表明，在深红红螺菌中参与细菌叶绿素和类胡萝卜素生物合成的基因的连锁顺序基本上与荚膜红细菌和球形红细菌中的排列相同。此外，在这些物种之间，编码光捕获和反应中心结构基因的puf和puh操纵子之间观察到的位置和相对距离也保守。光合作用基因簇的保守性意味着存在限制光合作用基因簇重排的结构或调控限制，或者光合作用基因簇可能在不同的光合细菌物种之间发生了横向转移。
