Coomber S A, Chaudhri M, Connor A, Britton G, Hunter C N
Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, UK.
Mol Microbiol. 1990 Jun;4(6):977-89. doi: 10.1111/j.1365-2958.1990.tb00670.x.
Four genes essential for bacteriochlorophyll biosynthesis were known to be encoded within a 45 kb region of the Rhodobacter sphaeroides genome, the boundaries of which are defined by puh and puf genes for reaction-centre and light-harvesting LH1 complexes. The cluster is represented by eight overlapping inserts cloned in the mobilizable vector pSUP202. We have used localized transposon Tn5 mutagenesis to characterize this cluster further; a total of 87 independent insertions were generated which identify nine genes for bacteriochlorophyll biosynthesis, six for carotenoid biosynthesis, and puhA encoding the reaction-centre H subunit. This work provides an essential framework for a detailed study of the structure and expression of genes for photosynthesis in this bacterium.
已知参与细菌叶绿素生物合成的四个基因位于球形红细菌基因组的一个45 kb区域内，该区域的边界由反应中心和捕光LH1复合体的puh和puf基因界定。该基因簇由克隆于可移动载体pSUP202中的八个重叠插入片段代表。我们利用定位转座子Tn5诱变进一步表征该基因簇；共产生了87个独立插入，鉴定出九个参与细菌叶绿素生物合成的基因、六个参与类胡萝卜素生物合成的基因，以及编码反应中心H亚基的puhA。这项工作为详细研究该细菌光合作用基因的结构和表达提供了重要框架。
