Burrus L W, Zuber M E, Lueddecke B A, Olwin B B
Department of Biochemistry, University of Wisconsin, Madison 53706.
Mol Cell Biol. 1992 Dec;12(12):5600-9. doi: 10.1128/mcb.12.12.5600-5609.1992.
The fibroblast growth factor (FGF) family consists of seven members whose activities are thought to be mediated by multiple receptors. Here we describe the cDNA cloning, expression, and characterization of a cysteine-rich FGF receptor (CFR) that is distinct from previously identified FGF receptors. The deduced amino acid sequence for CFR suggests that it is an integral membrane protein containing a large extracellular domain comprising 16 cysteine-rich repeated units and an intracellular domain of 13 amino acids. No reported sequences exhibit significant homologies to either the repeated extracellular motif or to the entire CFR amino acid sequence. Several CFR transcripts are present in embryonic chick tissue, suggesting that CFR undergoes alternate mRNA splicing or that related genes are present. Chinese hamster ovary cells transfected with the CFR cDNA express a 150-kDa polypeptide that binds FGF-1, FGF-2, and FGF-4 but does not bind several non-FGF family members. The high degree of evolutionary conservation among vertebrate CFRs and its ability to bind three different FGFs with high affinity suggest that this unique receptor plays an important role in FGF biology.
成纤维细胞生长因子（FGF）家族由七个成员组成，其活性被认为是由多种受体介导的。在此，我们描述了一种富含半胱氨酸的FGF受体（CFR）的cDNA克隆、表达及特性，该受体与先前鉴定的FGF受体不同。CFR的推导氨基酸序列表明它是一种整合膜蛋白，含有一个由16个富含半胱氨酸的重复单元组成的大细胞外结构域和一个13个氨基酸的细胞内结构域。没有报道的序列与重复的细胞外基序或整个CFR氨基酸序列表现出显著同源性。胚胎鸡组织中存在几种CFR转录本，这表明CFR经历了可变mRNA剪接，或者存在相关基因。用CFR cDNA转染的中国仓鼠卵巢细胞表达一种150 kDa的多肽，该多肽能结合FGF-1、FGF-2和FGF-4，但不结合几种非FGF家族成员。脊椎动物CFR之间高度的进化保守性及其与三种不同FGF高亲和力结合的能力表明，这种独特的受体在FGF生物学中起重要作用。
