Harleman J H, Koopman J P, Bremer-Mulder W G
Lab Anim. 1977 Oct;11(4):229-32. doi: 10.1258/002367777780936477.
2 sets of data are given of biotyping the Enterobacteriaceae from the faecal flora of SPF rats and cats housed with different isolation arrangements. The method is presented as an evaluation of the isolation efficiency of laboratory animals. Biotypes which were determined on almost every occasion belonged to the resident flora of the animals while those found occasionally represented contamination. Contaminating bacteria are cleared from an SPF breeding unit by colonization resistance and the continuous efflux of animals from the unit. A parameter of isolation efficacy is the number of different biotypes determined in time. With optimal isolation arrangements a stable bacterial flora of permanently colonized biotypes is obtained in the animals.
给出了两组关于不同隔离安排饲养的无特定病原体（SPF）大鼠和猫粪便菌群中肠杆菌科细菌生物分型的数据。该方法用于评估实验动物的隔离效率。几乎每次检测到的生物型都属于动物的固有菌群，而偶尔发现的生物型则代表污染。污染细菌可通过定植抗性和动物不断从该单元流出而从SPF繁殖单元中清除。隔离效果的一个参数是随时间确定的不同生物型的数量。通过最佳隔离安排，可在动物体内获得由永久定植生物型组成的稳定细菌菌群。
