Apperloo-Renkema H Z, Van der Waaij B D, Van der Waaij D
Laboratory for Medical Microbiology, State University of Groningen, The Netherlands.
Epidemiol Infect. 1990 Oct;105(2):355-61. doi: 10.1017/s0950268800047944.
In studies concerning the effect of antibiotics on faecal microflora, Colonization Resistance is an important parameter. Colonization Resistance correlates inversely with the number of different biotypes of Enterobacteriaceae isolated from faecal samples. Nine healthy volunteers were studied during 6 weeks, in order to determine the natural variation in the number of different biotypes of Enterobacteriaceae per faecal sample. The numbers of biotypes ranged from 1-15 per faecal sample, the mean number of biotypes varied between 2.6 and 7.3 different biotypes per faecal sample per healthy volunteer. Inter-individual variations of five biotypes in the mean number of biotypes per faecal sample are normal. We assessed the minimal number of faecal samples that should be taken for comprehensive biotyping so as to determine reliably the mean number of different biotypes representative for the Colonization Resistance of an individual. It was found that a minimum of four faecal samples was required.
在关于抗生素对粪便微生物群影响的研究中，定植抗力是一个重要参数。定植抗力与从粪便样本中分离出的肠杆菌科不同生物型的数量呈负相关。对9名健康志愿者进行了为期6周的研究，以确定每个粪便样本中肠杆菌科不同生物型数量的自然变化。每个粪便样本的生物型数量在1至15之间，每位健康志愿者每个粪便样本的生物型平均数量在2.6至7.3种不同生物型之间变化。每个粪便样本生物型平均数量中五种生物型的个体间差异是正常的。我们评估了为进行全面生物分型应采集的粪便样本的最小数量，以便可靠地确定代表个体定植抗力的不同生物型的平均数量。结果发现，至少需要四个粪便样本。
