PEPE F A
J Biophys Biochem Cytol. 1961 Dec;11(3):515-20. doi: 10.1083/jcb.11.3.515.
The preparation of antimyosin conjugated with mercury and fluorescein is described. The mercury was introduced to permit visualization of the antibody in the electron microscope. An organic mercurial, tetraacetoxymercuriarsanilic acid, was prepared and coupled to the antibody through the diazonium salt. The fluorescein was coupled through the isocyanate by a modification of the procedure described by Coons and Kaplan. The antibody conjugate retained its specificity of reaction with the tissue antigen. This was demonstrated by the staining pattern obtained in fluorescence microscopy.
本文描述了与汞和荧光素偶联的抗肌球蛋白的制备方法。引入汞是为了在电子显微镜下观察抗体。制备了一种有机汞化合物，四乙酰氧基汞基对氨基苯磺酸，并通过重氮盐与抗体偶联。荧光素通过异氰酸酯，采用孔斯（Coons）和卡普兰（Kaplan）所描述方法的改进方法进行偶联。抗体偶联物保留了其与组织抗原反应的特异性。这通过荧光显微镜下获得的染色模式得到了证明。
