使用25千道尔顿外膜蛋白和粗糙脂多糖特异性单克隆抗体通过乳胶凝集试验快速鉴定粗糙布鲁氏菌分离株。
Rapid identification of rough Brucella isolates by a latex coagglutination assay with the 25-kilodalton outer membrane protein and rough-lipopolysaccharide-specific monoclonal antibodies.
作者信息
Bowden R A, Verger J M, Grayon M, Cloeckaert A
机构信息
Laboratoire de Pathologie Infectieuse et Immunologie, INRA, Centre de Recherches de Tours, Nouzilly, France.
出版信息
Clin Diagn Lab Immunol. 1997 Sep;4(5):611-4. doi: 10.1128/cdli.4.5.611-614.1997.
Abstract
A latex coagglutination assay was developed to identify rough (R) isolates of Brucella. Latex beads were coated, via protein A, with either an anti-Brucella rough-lipopolysaccharide (R-LPS) monoclonal antibody (MAb) or an anti-Brucella 25-kDa outer membrane protein (Omp25) MAb. Slide agglutination tests were done for 68 strains of Brucella spp., including type strains of all biovars as well as field isolates. Latex beads coated with MAb to R-LPS coagglutinated only R strains, whereas latex beads coated with MAb to Omp25 coagglutinated all the R Brucella isolates except Brucella ovis. Coagglutination was easier to read than agglutination with rabbit R-Brucella-specific antiserum. Thus, this assay accurately differentiates B. ovis from other R Brucella isolates. The latex coagglutination assay can substitute, to advantage, for the current anti-Brucella (R) rabbit monospecific serum.
摘要
开发了一种乳胶凝集试验来鉴定布鲁氏菌粗糙（R）型分离株。通过蛋白A将乳胶珠包被上抗布鲁氏菌粗糙脂多糖（R-LPS）单克隆抗体（MAb）或抗布鲁氏菌25 kDa外膜蛋白（Omp25）单克隆抗体。对68株布鲁氏菌属菌株进行了玻片凝集试验，包括所有生物型的模式菌株以及野外分离株。包被有抗R-LPS单克隆抗体的乳胶珠仅与R型菌株发生凝集反应，而包被有抗Omp25单克隆抗体的乳胶珠与除绵羊布鲁氏菌外的所有R型布鲁氏菌分离株发生凝集反应。凝集反应比用兔抗R型布鲁氏菌特异性抗血清进行的凝集反应更易于观察。因此，该试验能准确地区分绵羊布鲁氏菌与其他R型布鲁氏菌分离株。乳胶凝集试验可以有效地替代目前的抗布鲁氏菌（R）兔单特异性血清。
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