PROTHERO J W, BURTON A C
Biophys J. 1962 Mar;2(2 Pt 1):213-22. doi: 10.1016/s0006-3495(62)86850-7.
Two previous communications dealt with the nature of the motion and the resistance to flow in capillary blood flow. In this final paper measurements are reported of the pressure required to force mammalian erythrocytes through pores having a diameter less than the cells. The cells, from fresh human or dog blood, were resuspended in acid citrate dextrose solutions. The final suspensions (about 1,000 times more dilute than whole blood) were immediately emptied into a millipore filter apparatus and the rate of filtration was measured. Filters having pore diameters of 5.0 and 3.0 microns were employed. The cellular concentration of samples of the original suspensions and of the filtrate was determined. It was observed that the rate of filtration decreased rapidly initially and then became constant. In the ;steady state' the cellular concentration of samples of the filtrate was found to be approximately equal to that of the original suspension. A simple theory is presented which adequately describes the flow of a suspension through such filters. It is concluded that mammalian erythrocytes, particularly human and dog cells, will pass steadily, without hemolysis, through pores 5.0 or 3.0 microns in diameter under pressures of 4 cm of water or less.
之前的两篇通讯讨论了毛细血管血流中的运动性质和对血流的阻力。在这篇最后的论文中，报告了迫使哺乳动物红细胞通过直径小于细胞的孔隙所需压力的测量结果。取自新鲜人血或狗血的细胞被重悬于酸性枸橼酸盐葡萄糖溶液中。最终的悬浮液（比全血稀释约1000倍）立即倒入微孔过滤装置中，并测量过滤速率。使用了孔径为5.0微米和3.0微米的过滤器。测定了原始悬浮液和滤液样品的细胞浓度。观察到过滤速率最初迅速下降，然后变得恒定。在“稳定状态”下，发现滤液样品的细胞浓度与原始悬浮液的细胞浓度大致相等。提出了一个简单的理论，该理论充分描述了悬浮液通过此类过滤器的流动。得出的结论是，哺乳动物红细胞，特别是人红细胞和狗红细胞，在4厘米水柱或更低的压力下，将稳定地通过直径为5.0或3.0微米的孔隙，且不会发生溶血。
