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暴露于极低频电磁场的人白血病HL - 60细胞对3H - 尿苷的摄取

3H-uridine uptake in human leukemia HL-60 cells exposed to extremely low frequency electromagnetic fields.

作者信息

Azadniv M, Miller M W

机构信息

Department of Biophysics, School of Medicine and Dentistry, University of Rochester, New York 14642.

出版信息

Biochem Biophys Res Commun. 1992 Nov 30;189(1):437-44. doi: 10.1016/0006-291x(92)91577-d.

DOI:10.1016/0006-291x(92)91577-d
PMID:1449495
Abstract

Human leukemia HL-60 cells in vitro were exposed or sham-exposed to a 60 Hz electric (3.40 mV/m) or magnetic 10 G (1 muT) field for 5 hr in an attempt to replicate the results of a previously published report (1) of increased uptake of [3H] uridine in response to similar exposure conditions. The results of the present experiments indicated no treatment effect of the fields on uridine incorporation. The ability to detect differences in [3H] uridine uptake were confirmed in a negative control experiment. A 'negative control' experiment demonstrated a statistically significant increase in 3H-uridine uptake for cells at 37 degrees C relative to those at 4 degrees C.

摘要

将人白血病HL - 60细胞在体外暴露于或假暴露于60 Hz电场(3.40 mV/m)或10 G(1 μT)磁场中5小时,试图重复之前发表的一份报告(1)的结果,该报告称在类似暴露条件下[3H]尿苷摄取增加。本实验结果表明,这些场对尿苷掺入没有处理效应。在阴性对照实验中证实了检测[3H]尿苷摄取差异的能力。一项“阴性对照”实验表明,相对于4℃的细胞,37℃的细胞3H - 尿苷摄取有统计学上的显著增加。

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