Greene J J, Skowronski W J, Mullins J M, Nardone R M, Penafiel M, Meister R
Department of Biology, Catholic University of America, Washington, DC 20064.
Biochem Biophys Res Commun. 1991 Jan 31;174(2):742-9. doi: 10.1016/0006-291x(91)91480-z.
The relative effects of the electric and magnetic field components of extremely low frequency electromagnetic radiation (ELF) on transcription were examined in human leukemia HL-60 cells. Delineation of the individual field contributions was achieved by irradiating cells in separate concentric compartments of a culture dish within a solenoid chamber. This exposure system produced a homogeneous magnetic field with a coincident electric field whose strength varied directly with distance from the center of the culture dish. Irradiation of HL-60 cells with sine wave ELF at 60 Hz and a field strength of 10 Gauss produced a transient increase in the transcriptional rates which reached a maximum of 50-60% enhancement at 30-120 minutes of irradiation and declined to near basal levels by 18 hours. Comparison of transcription responses to ELF of cells in different concentric compartments revealed that the transcriptional effects were primarily the result of the electric field component with little or no contribution from the magnetic field.
在人白血病HL-60细胞中研究了极低频电磁辐射（ELF）的电场和磁场成分对转录的相对影响。通过在螺线管腔内培养皿的单独同心隔室中照射细胞，实现了对各个场贡献的描绘。该暴露系统产生了一个均匀的磁场以及一个与之重合的电场，其强度随距培养皿中心的距离直接变化。用60 Hz的正弦波ELF和10高斯的场强照射HL-60细胞，导致转录速率出现短暂增加，在照射30 - 120分钟时达到最大增强50 - 60%，并在18小时时降至接近基础水平。对不同同心隔室中细胞对ELF的转录反应进行比较发现，转录效应主要是电场成分的结果，磁场的贡献很小或没有贡献。
