Aspeslet L J, Baker G B, Coutts R T, Mousseau D D
Department of Psychiatry, University of Alberta, Edmonton, Canada.
Biochem Pharmacol. 1992 Nov 3;44(9):1894-7. doi: 10.1016/0006-2952(92)90088-z.
A novel assay procedure has been developed that allows for the separation and quantification of the enantiomers of the monoamine oxidase inhibitor tranylcypromine (TCP) in brain and liver of rats. The analytical method involves extraction of the drug from rat tissue with an organic solvent. TCP is then derivatized with S-(-)-N-(trifluoroacetyl)-prolyl chloride to allow gas chromatographic analysis of the resulting diastereoisomers. Conditions for analysis by a gas chromatograph equipped with a nitrogen-phosphorus detector and a capillary column are described. The method has been applied to the separation and quantification of the enantiomers of TCP in samples of brain and liver of rats that had been injected with this drug alone and after pretreatment with iprindole, a drug known to block aromatic ring hydroxylation.
已开发出一种新的检测方法，可对大鼠脑和肝中单胺氧化酶抑制剂反苯环丙胺（TCP）的对映体进行分离和定量。该分析方法包括用有机溶剂从大鼠组织中提取药物。然后，TCP用S-(-)-N-(三氟乙酰基)-脯氨酰氯进行衍生化，以便对所得非对映异构体进行气相色谱分析。描述了配备氮磷检测器和毛细管柱的气相色谱仪的分析条件。该方法已应用于单独注射该药物以及用已知可阻断芳香环羟基化的药物伊普吲哚预处理后的大鼠脑和肝样品中TCP对映体的分离和定量。
