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成熟小鼠卵母细胞暴露于可见光后进行体外受精、发育及着床。

In vitro fertilization, development, and implantation after exposure of mature mouse oocytes to visible light.

作者信息

Barlow P, Puissant F, Van der Zwalmen P, Vandromme J, Trigaux P, Leroy F

机构信息

IVF Clinic and Human Reproduction Research Unit, Saint Pierre Hospital, Free University of Brussels, Belgium.

出版信息

Mol Reprod Dev. 1992 Nov;33(3):297-302. doi: 10.1002/mrd.1080330310.

DOI:10.1002/mrd.1080330310
PMID:1449795
Abstract

Mature mouse oocytes were exposed prior to in vitro fertilization to visible light during 1, 2, or 4 hr at an intensity of 4,000 lux. Compared to controls cultured under identical conditions but protected from light, exposed eggs did not show any significant modification of cleavage speed and rate. After transfer of blastocysts obtained in vitro in uteri of pseudopregnant females, the implantation rate and the proportion of normal fetuses were not found to be different in relation to preliminary light exposure of oocytes fertilized and cultured in vitro.

摘要

成熟的小鼠卵母细胞在体外受精前,于4000勒克斯的强度下暴露于可见光下1、2或4小时。与在相同条件下培养但避光的对照组相比,暴露的卵母细胞在分裂速度和速率上没有显示出任何显著变化。将体外获得的囊胚移植到假孕雌性的子宫后,未发现与体外受精和培养的卵母细胞预先光照相关的着床率和正常胎儿比例有差异。

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