Zhao Shui-ping, Zhang Da-qing
Department of Cardiology, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, PR China.
Clin Chim Acta. 2003 Oct;336(1-2):103-8. doi: 10.1016/s0009-8981(03)00335-8.
Interleukin-6 (IL-6) is secreted by adipocytes and may be involved in atherosclerosis. Few studies have assessed the influence of statins on IL-6 secretion in adipocytes.
Rabbits on high-cholesterol diets were randomly given either atorvastatin 1.5 mg/kg day(-1) (n=5) or starch (n=5) for 2 weeks. Subcutaneous adipose was collected for adipocytes culture. IL-6 concentrations in plasma and adipocytes culture supernatant were measured by ELISA. RT-PCR was used to evaluate peroxisome proliferator-activated receptor (PPAR) gamma mRNA expression. The in vitro effect of atorvastatin on IL-6 production in adipocytes was observed.
Two weeks atorvastatin treatment resulted in significant reduction of circulating IL-6 concentrations, which was associated with IL-6 secretion in adipocytes (r=0.849, P<0.01). Meanwhile mRNA expression of PPARgamma in adipocytes was intimately related to the IL-6 secretion in adipocytes (r=-0.900, P<0.01). Atorvastatin induced the decreased IL-6 secretion in dose-dependent manner.
Atorvastatin can inhibit IL-6 secretion in adipocytes possibly through upregulating PPARgamma, which may help to explain the anti-inflammatory effects of statins use.
白细胞介素-6（IL-6）由脂肪细胞分泌，可能参与动脉粥样硬化的发生。很少有研究评估他汀类药物对脂肪细胞中IL-6分泌的影响。
给高胆固醇饮食的兔子随机每日给予阿托伐他汀1.5mg/kg（n = 5）或淀粉（n = 5），持续2周。收集皮下脂肪用于脂肪细胞培养。采用酶联免疫吸附测定法（ELISA）检测血浆和脂肪细胞培养上清液中IL-6的浓度。运用逆转录-聚合酶链反应（RT-PCR）评估过氧化物酶体增殖物激活受体（PPAR）γ mRNA的表达。观察阿托伐他汀对脂肪细胞中IL-6产生的体外作用。
阿托伐他汀治疗2周导致循环中IL-6浓度显著降低，这与脂肪细胞中IL-6的分泌相关（r = 0.849，P < 0.01）。同时，脂肪细胞中PPARγ的mRNA表达与脂肪细胞中IL-6的分泌密切相关（r = -0.900，P < 0.01）。阿托伐他汀以剂量依赖的方式诱导IL-6分泌减少。
阿托伐他汀可能通过上调PPARγ抑制脂肪细胞中IL-6的分泌，这可能有助于解释他汀类药物使用的抗炎作用。
