Zhao Shui-ping, Wu Zhi-hong
Department of Cardiology, The Second Xiangya Hospital of Central South University, middle Ren-Min road No.86, Changsha, Hunan 410011, PR China.
Clin Chim Acta. 2005 Oct;360(1-2):133-40. doi: 10.1016/j.cccn.2005.04.021.
Leptin may play an important role in the development of atherosclerosis. We evaluated the effect of atorvastatin on leptin secretion in vivo and in vitro.
Sixteen rabbits fed with high-cholesterol diet for 8 weeks were randomly divided into 2 groups: (1) high cholesterol diet for 6 weeks (n=8), and (2) the same cholesterol diet plus atorvastatin (2.5 mg/kg/day) for 6 weeks (n=8). A control group (n=5) was fed with normal diet for 14 weeks. Subcutaneous adipose was collected for RNA analysis. The direct effect of atorvastatin on leptin release was assayed in primary rabbit adipocytes. Leptin levels in serum and adipocytes culture supernatant were measured by ELISA. RT-PCR was used to evaluate leptin mRNA expressions in adipose and adipocytes.
Compared with control group, rabbits fed with high cholesterol diet showed higher levels of serum total cholesterol, LDL cholesterol and leptin, all of which were significantly reduced by atorvastatin treatment. Leptin mRNA expression of adipose was significant lower in rabbits treated with atorvastatin than those fed with high cholesterol diet continuously (0.81+/-0.31 vs. 1.23+/-0.36, P<0.05). Atorvastatin dose-dependently inhibited leptin secretion and mRNA expression in cultured adipocytes.
Atorvastatin can inhibit leptin release and mRNA expression, and reduces serum leptin level in hypercholesterolemic rabbits.
瘦素可能在动脉粥样硬化的发展中起重要作用。我们评估了阿托伐他汀在体内和体外对瘦素分泌的影响。
将16只喂食高胆固醇饮食8周的兔子随机分为2组：（1）高胆固醇饮食6周（n = 8），以及（2）相同的胆固醇饮食加阿托伐他汀（2.5毫克/千克/天）6周（n = 8）。一个对照组（n = 5）喂食正常饮食14周。收集皮下脂肪进行RNA分析。在原代兔脂肪细胞中测定阿托伐他汀对瘦素释放的直接影响。通过ELISA测量血清和脂肪细胞培养上清液中的瘦素水平。RT-PCR用于评估脂肪和脂肪细胞中瘦素mRNA的表达。
与对照组相比，喂食高胆固醇饮食的兔子血清总胆固醇、低密度脂蛋白胆固醇和瘦素水平更高，而阿托伐他汀治疗可显著降低所有这些指标。阿托伐他汀治疗的兔子脂肪中的瘦素mRNA表达明显低于持续喂食高胆固醇饮食的兔子（0.81±0.31对1.23±0.36，P<0.05）。阿托伐他汀在培养的脂肪细胞中剂量依赖性地抑制瘦素分泌和mRNA表达。
阿托伐他汀可抑制瘦素释放和mRNA表达，并降低高胆固醇血症兔子的血清瘦素水平。
