Nandhini Thirugnanam Anitha, Anuradha Carani Venkataraman
Department of Biochemistry, Faculty of Science, Annamalai University, Annamalainagar-608 002, Tamil Nadu, India.
Clin Chim Acta. 2003 Oct;336(1-2):129-35. doi: 10.1016/s0009-8981(03)00337-1.
Supplementation of taurine, a sulfur containing amino acid has been found to be beneficial in counteracting oxidative stress and in preventing experimental diabetic neuropathy, nephropathy and retinopathy. Taurine has its own capacity to prevent the suppression of membrane-bound Na(+)/K(+)ATPase activity and prevent Ca(2+) overload. This study was undertaken to test whether taurine can reduce lipid peroxidation and glycosylation and can increase the Na(+)/K(+)- and Ca(2+)-ATPase activities in high glucose-treated red blood cells (RBC).
Washed normal human RBC were incubated in phosphate-buffered saline with normal (6 mmol/l) or high glucose concentrations (45 mmol/l), with and without 50-150 micromol/l taurine in a shaking water bath at 37 degrees C for 24 h. Lipid peroxidation, glycated hemoglobin, glucose utilization and Na(+)/K(+)- and Ca(2+)-ATPase activities were determined in the glucose-treated human RBC.
Taurine significantly lowered the level of glycated hemoglobin (GHb) and lipid peroxidation in RBC exposed to high glucose concentrations. Stimulation of glucose utilization by RBC was significant in the presence of taurine both in normal and high glucose-treated RBC. The activities of Na(+)/K(+)- and Ca(2+)-ATPases in RBC membranes were significantly lowered in high glucose-treated RBC. Taurine treatment significantly prevented the reduction in activities of Na(+)/K(+)- and Ca(2+)-ATPases activities in high glucose-treated RBC.
The results show that taurine is important for the physiological functions of RBCs and the effects of taurine on glucose-treated RBC may have potential therapeutic relevance in diabetes.
已发现补充含硫氨基酸牛磺酸有助于对抗氧化应激，并预防实验性糖尿病性神经病变、肾病和视网膜病变。牛磺酸自身具有防止膜结合型钠/钾ATP酶活性受抑制以及防止钙超载的能力。本研究旨在测试牛磺酸是否能降低高糖处理的红细胞（RBC）中的脂质过氧化和糖基化水平，并提高钠/钾-ATP酶和钙-ATP酶的活性。
将洗涤后的正常人红细胞置于含正常（6 mmol/L）或高糖浓度（45 mmol/L）的磷酸盐缓冲盐水中，在37℃的振荡水浴中分别添加和不添加50 - 150 μmol/L牛磺酸孵育24小时。测定经葡萄糖处理的人红细胞中的脂质过氧化、糖化血红蛋白、葡萄糖利用情况以及钠/钾-ATP酶和钙-ATP酶的活性。
牛磺酸显著降低了暴露于高糖浓度下的红细胞中糖化血红蛋白（GHb）的水平和脂质过氧化程度。在正常和高糖处理的红细胞中，牛磺酸均能显著促进红细胞对葡萄糖的利用。高糖处理的红细胞中红细胞膜上钠/钾-ATP酶和钙-ATP酶的活性显著降低。牛磺酸处理显著防止了高糖处理的红细胞中钠/钾-ATP酶和钙-ATP酶活性的降低。
结果表明，牛磺酸对红细胞的生理功能很重要，牛磺酸对经葡萄糖处理的红细胞的作用可能在糖尿病治疗中具有潜在的相关性。
