McMullen Michael D
U.S. Department of Agriculture-Agricultural Research Service, Plant Genetics Research Unit, and Plant Science Unit, University of Missouri, Columbia, MO, USA.
Methods Mol Biol. 2003;236:141-54. doi: 10.1385/1-59259-413-1:141.
Quantitative trait locus analysis has been a mainstay approach for obtaining a genetic description of complex agronomic traits for plants. What is sometimes overlooked is the role quantitative trait locus (QTL) analysis can play in identifying genes that underlay complex traits. In this chapter, I will describe the basic steps required to conduct QTL analysis in crop plants. This process involves choices by the investigator on type of population to be studied, molecular marker system to be used to genotype the population, and methods for QTL analysis. Examples of cloned genes, first identified as QTL, are also given to persuade the reader of the power of QTL analysis to discover genes controlling traits and phenotypes.
数量性状基因座分析一直是获取植物复杂农艺性状遗传描述的主要方法。有时被忽视的是数量性状基因座（QTL）分析在鉴定复杂性状潜在基因方面所能发挥的作用。在本章中，我将描述在农作物中进行QTL分析所需的基本步骤。这个过程涉及研究者在要研究的群体类型、用于对群体进行基因分型的分子标记系统以及QTL分析方法等方面的选择。还给出了最初被鉴定为QTL的克隆基因实例，以让读者相信QTL分析在发现控制性状和表型的基因方面的强大作用。
