Whitt Sherry R, Buckler Edward S
U.S. Department of Agriculture-Agricultural Research Service, Raleigh, NC, USA.
Methods Mol Biol. 2003;236:123-40. doi: 10.1385/1-59259-413-1:123.
Genomics has developed a wide range of tools to identify genes that play roles in specific pathways. However, relating individual genes and alleles to agronomic traits is still quite challenging. We describe how association analysis can be used to relate natural variation at candidate genes with agronomic phenotypes. Association approaches in plants can provide very high resolution and can evaluate a wide range of alleles rapidly. We discuss issues related to experimental design, germplasm sample, molecular assay, population structure, and statistical analysis necessary for association analysis in plants.
基因组学已经开发出了一系列广泛的工具来识别在特定途径中发挥作用的基因。然而，将单个基因和等位基因与农艺性状联系起来仍然颇具挑战性。我们描述了如何利用关联分析将候选基因的自然变异与农艺表型联系起来。植物中的关联分析方法可以提供非常高的分辨率，并且能够快速评估多种等位基因。我们讨论了与植物关联分析所需的实验设计、种质样本、分子检测、群体结构和统计分析相关的问题。
