Anisimov Vladimir N, Khavinson Vladimir Kh, Popovich Irina G, Zabezhinski Mark A, Alimova Irina N, Rosenfeld Svetlana V, Zavarzina Natalia Yu, Semenchenko Anna V, Yashin Anatoli I
Department of Carcinogenesis and Oncogerontology, NN Petrov Research Institute of Oncology, Pesochny-2, St. Petersburg, 197758, Russia.
Biogerontology. 2003;4(4):193-202. doi: 10.1023/a:1025114230714.
From the age of 3 months until their natural deaths, female outbred Swiss-derived SHR mice were subcutaneously injected on 5 consecutive days every month with 0.1 ml of normal saline (control) or with 1.0 microg/mouse (approximately 30-40 microg/kg) of tetrapeptide Epitalon (Ala-Glu-Asp-Gly) dissolved in 0.1 ml saline. There were 54 mice in each group. The results of this study show that treatment with Epitalon did not influence food consumption, body weight or mean life span of mice. However, it slowed down the age-related switching-off of estrous function and decreased the frequency of chromosome aberrations in bone marrow cells (by 17.1%, P<0.05). It also increased by 13.3% the life span of the last 10% of the survivors (P<0.01) and by 12.3% the maximum life span in comparison with the control group. We also found that treatment with Epitalon did not influence total spontaneous tumor incidence, but inhibited the development of leukemia (6.0-fold), as compared with the control group. The data obtained suggest a geroprotector activity of Epitalon and the safety of its long-term administration in mice.
从3个月龄直至自然死亡，每月连续5天对雌性远交瑞士种源SHR小鼠皮下注射0.1毫升生理盐水（对照组）或溶解于0.1毫升生理盐水中的1.0微克/只（约30 - 40微克/千克）四肽Epitalon（丙氨酸 - 谷氨酸 - 天冬氨酸 - 甘氨酸）。每组有54只小鼠。本研究结果表明，用Epitalon治疗不影响小鼠的食物摄入量、体重或平均寿命。然而，它减缓了与年龄相关的发情功能关闭，并降低了骨髓细胞中染色体畸变的频率（降低17.1%，P<0.05）。与对照组相比，它还使最后10%存活者的寿命延长了13.3%（P<0.01），使最大寿命延长了12.3%。我们还发现，用Epitalon治疗不影响总的自发肿瘤发生率，但与对照组相比，抑制了白血病的发生（6.0倍）。所获得的数据表明Epitalon具有老年保护活性及其在小鼠中长期给药的安全性。
