Poopathi Subbiah, Archana B
Unit of Microbiology and Immunology, Vector Control Research Centre (ICMR) Ministry of Health and Family Welfare, Government of India, Indira Nagar, Pondicherry 60 5006, India.
Indian J Exp Biol. 2012 Jan;50(1):65-71.
Optimization of chicken feather (CF) based culture medium for the production of Bacillus thuringiensis subsp. israelensis (Bti) biomass in combination with the agro industrial by-product (coconut cake, CC) and manganese chloride (MnCl2) has been evaluated. The biomass yield of Bti spore/crystal toxin was highest (12.06 g/L) from the test medium (CF+CC+MnCl2) compared to the reference medium (Luria Bertani, LB). Toxicity assay with Bti produced from the test medium against mosquito vectors (Culex quinquefasciatus, Anopheles stephensi and Aedes aegypti) was also satisfactory and results were comparable with bacteria produced from LB. The results suggest that Bti can be produced to the maximum extent possible as a potential mosquitocidal activity as suggested by the test medium (CF+CC+MnCl2).
评估了以鸡毛（CF）为基础的培养基与农用工业副产品（椰子饼，CC）和氯化锰（MnCl₂）组合用于生产苏云金芽孢杆菌以色列亚种（Bti）生物质的优化情况。与参考培养基（Luria Bertani，LB）相比，测试培养基（CF + CC + MnCl₂）中Bti孢子/晶体毒素的生物质产量最高（12.06 g/L）。用测试培养基生产的Bti对蚊媒（致倦库蚊、斯氏按蚊和埃及伊蚊）进行的毒性试验也令人满意，结果与LB培养基生产的细菌相当。结果表明，如测试培养基（CF + CC + MnCl₂）所示，Bti作为一种潜在的杀蚊活性物质可以在最大程度上进行生产。
