Löbler M, Hirsch A M
Department of Biology, University of California, Los Angeles 90024.
Plant Mol Biol. 1992 Nov;20(4):733-6. doi: 10.1007/BF00046457.
We have found an alfalfa cDNA clone that encodes an acidic leghemoglobin. To date, 14 alfalfa leghemoglobin clones have been identified. Five different leghemoglobin 'components' have been biochemically defined on the basis of their pI. A higher-resolution comparison, provided by sequence data analysis, identifies six leghemoglobin 'classes'. All 14 leghemoglobins are assigned to the six 'classes', which can be distributed among the five leghemoglobin 'components'. The newly identified leghemoglobin is the only member of a sixth 'class' of leghemoglobins, and it also is the only member of one of the acidic leghemoglobin 'components' IV or V.
我们发现了一个编码酸性豆血红蛋白的苜蓿cDNA克隆。迄今为止,已鉴定出14个苜蓿豆血红蛋白克隆。根据它们的等电点,已在生物化学上定义了五种不同的豆血红蛋白“组分”。序列数据分析提供的更高分辨率比较确定了六种豆血红蛋白“类别”。所有14种豆血红蛋白都被归入这六种“类别”,它们可以分布在五种豆血红蛋白“组分”中。新鉴定出的豆血红蛋白是豆血红蛋白第六种“类别”的唯一成员,并且它也是酸性豆血红蛋白“组分”IV或V之一的唯一成员。