Kiss G B, Vincze E, Végh Z, Tóth G, Soós J
Institute of Genetics, Hungarian Academy of Sciences, Szeged.
Plant Mol Biol. 1990 Apr;14(4):467-75. doi: 10.1007/BF00027493.
A new nodule-specific gene, Nms-25 (nodulin-25), was identified in cDNA clones isolated from a nodule-specific cDNA library of Medicago sativa. The first transcript of this gene appeared 9 days after inoculation of the roots with Rhizobium meliloti. The time of expression and the quantity of the transcripts of the Nms-25 gene was similar to that of leghemoglobin genes suggesting a similar regulation. A protein of 246 amino acids could be deduced from a full-length cDNA clone. The first 24 amino acids at the N-terminal end of this protein formed a signal sequence which might direct membrane transport into the peribacteroid space. Using different predictive methods the signal sequence cleaved protein was tentatively predicted to be a water-soluble enzyme, but not hydrolase.
从紫花苜蓿根瘤特异性cDNA文库中分离得到的cDNA克隆中，鉴定出一个新的根瘤特异性基因Nms-25（根瘤蛋白-25）。在用苜蓿根瘤菌接种根部9天后，该基因的首个转录本出现。Nms-25基因转录本的表达时间和数量与豆血红蛋白基因相似，表明存在相似的调控机制。从一个全长cDNA克隆中可以推断出一种由246个氨基酸组成的蛋白质。该蛋白质N端的前24个氨基酸形成一个信号序列，可能指导其通过膜转运进入类菌体周隙。使用不同的预测方法，初步预测该信号序列切割后的蛋白质是一种水溶性酶，但不是水解酶。
