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紫花苜蓿(Medicago sativa L.)胞质L5核糖体蛋白编码cDNA的克隆与表达

Cloning and expression of a cDNA encoding a cytoplasmic L5 ribosomal protein from alfalfa (Medicago sativa L.).

作者信息

Asemota O, Breda C, Sallaud C, el Turk J, de Kozak I, Buffard D, Esnault R, Kondorosi A

机构信息

Institut des Sciences Végétales, CNRS, Gif sur Yvette, France.

出版信息

Plant Mol Biol. 1994 Nov;26(4):1201-5. doi: 10.1007/BF00040700.

DOI:10.1007/BF00040700
PMID:7811977
Abstract

A cDNA encoding a putative cytoplasmic ribosomal protein L5 from alfalfa (MsRL5), the first sequence from higher plants, has been characterized. The derived amino acid sequence of 181 residues contains the L5 signature, is 72.2% identical to yeast ribosomal L5 and shares high identity with other RL5 peptides from eukaryotic origin. The sequence does not contain any signal or transit peptide and therefore might be cytoplasmic. In all alfalfa organs examined MsRL5 transcripts were detected at approximately equal levels.

摘要

已经鉴定出一种来自苜蓿的编码假定细胞质核糖体蛋白L5(MsRL5)的cDNA,这是高等植物中的首个此类序列。推导得到的由181个残基组成的氨基酸序列包含L5特征序列,与酵母核糖体L5的同源性为72.2%,并且与其他真核来源的RL5肽具有高度同源性。该序列不包含任何信号肽或转运肽,因此可能存在于细胞质中。在所检测的苜蓿所有器官中,MsRL5转录本的检测水平大致相同。

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Mol Plant Microbe Interact. 1993 Sep-Oct;6(5):655-64. doi: 10.1094/mpmi-6-655.
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Nucleic Acids Res. 1984 Nov 26;12(22):8295-312. doi: 10.1093/nar/12.22.8295.
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The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene.
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Nucleic Acids Res. 1983 May 11;11(9):2599-616. doi: 10.1093/nar/11.9.2599.
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