Wang S, Feng N, Li J, Yang Y, Liu X
Cancer Institute, CAMS, Beijing.
Chin Med Sci J. 1992 Jun;7(2):82-5.
In the present study, we have compared the distribution in various phases, DNA content, cell survival and ultrastructure of HeLa S3 cells after irradiation and/or hyperthermia. After 220 KV X-ray irradiation, the Do of the survival curve was 0.94 Gy, Dq was 1.3 Gy, and N was 4.26. After Ir-192 gamma-ray irradiation, the Do of the survival curve was 2.26 Gy, Dq was 3.9 Gy, and N was 5.7. The Do of the survival curve of HeLa S3 cells after treatment at 43.5 degrees C and 44 degrees C was 2.2 min and 1.6 min, respectively. Ultrastructural changes were also observed. The marked increase of the DNA content after 6 Gy irradiation corresponded with changes of distribution in various phases, which indicated a delay in the G2 + M phase. The survival fraction after 6 Gy irradiation was less than 1%. The changes of cell cycle distribution after Ir-192 irradiation were similar to those seen after X-ray exposure. The delay of G2 + M phase after hyperthermia was dose-dependent. An obvious delay of the G2 + M phases was also observed at 24 h after treatment with X-rays plus hyperthermia.
在本研究中，我们比较了HeLa S3细胞在照射和/或热疗后的各期分布、DNA含量、细胞存活率及超微结构。220 kV X线照射后，存活曲线的Do为0.94 Gy，Dq为1.3 Gy，N为4.26。铱-192 γ线照射后，存活曲线的Do为2.26 Gy，Dq为3.9 Gy，N为5.7。43.5℃和44℃处理后HeLa S3细胞存活曲线的Do分别为2.2分钟和1.6分钟。还观察到超微结构变化。6 Gy照射后DNA含量显著增加，与各期分布变化一致，表明G2 + M期延迟。6 Gy照射后的存活分数小于1%。铱-192照射后细胞周期分布变化与X线照射后相似。热疗后G2 + M期延迟呈剂量依赖性。X线加用热疗处理24小时后也观察到G2 + M期明显延迟。
