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Presteady state kinetic analysis of riboflavin synthase.

作者信息

Illarionov Boris, Haase Ilka, Bacher Adelbert, Fischer Markus, Schramek Nicholas

机构信息

Lehrstuhl für Organische Chemie und Biochemie, Technische Universität Munich, Lichtenbergstrasse 4, D-85747 Garching, Germany.

出版信息

J Biol Chem. 2003 Nov 28;278(48):47700-6. doi: 10.1074/jbc.M305050200. Epub 2003 Sep 22.

DOI:10.1074/jbc.M305050200
PMID:14504292
Abstract

Riboflavin synthase catalyzes a mechanistically complex dismutation affording riboflavin and 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione from 6,7-dimethyl-8-ribityllumazine. The kinetics of the enzyme from Escherichia coli were studied under single turnover conditions. Stopped flow as well as quenched flow experiments documented the transient formation of a pentacyclic reaction intermediate. No other transient species were sufficiently populated to allow detection. The data are best described by a sequence of one second order and one first order reaction.

摘要

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