Anil S, Samaranayake L P
Oral Bio-Sciences, Faculty of Dentistry, University of Hong Kong, Hong Kong.
Chemotherapy. 2003 Sep;49(5):243-7. doi: 10.1159/000072448.
Although the phospholipase activity is considered a potential virulence determinant of the pathogenic Candida species, the effect of antimycotics on this attribute is not known. Hence we evaluated the phospholipase activity in 10 isolates each of Candida albicans and Candida tropicalis, after their exposure to antifungals.
The impact of antimycotics on phospholipase activity was also assessed after exposure of the isolates to sub-minimum inhibitory concentrations of nystatin, amphotericin B and fluconazole.
All Candida isolates investigated exhibited phospholipase activity (Pz). In general C. ALBICANS showed relatively higher P(z) activity than C. tropicalis , and exposure of the isolates to antimycotics led to a significant (p < 0.05) reduction in the phospholipase activity. Nystatin and amphotericin B, but not fluconazole, significantly reduced the phospholipase activity of both Candida species.
These observations, while confirming the higher virulence of C. albicans relative to C. tropicalis, demonstrate for the first time the effect of antifungal agents on extracellular phospholipases of these common opportunistic pathogens.
尽管磷脂酶活性被认为是致病性念珠菌潜在的毒力决定因素，但抗真菌药物对该特性的影响尚不清楚。因此，我们在白色念珠菌和热带念珠菌的10个分离株暴露于抗真菌药物后，评估了它们的磷脂酶活性。
在分离株暴露于制霉菌素、两性霉素B和氟康唑的亚最低抑菌浓度后，还评估了抗真菌药物对磷脂酶活性的影响。
所有研究的念珠菌分离株均表现出磷脂酶活性（Pz）。一般来说，白色念珠菌的P(z)活性比热带念珠菌相对更高，并且分离株暴露于抗真菌药物导致磷脂酶活性显著降低（p < 0.05）。制霉菌素和两性霉素B，但不是氟康唑，显著降低了两种念珠菌的磷脂酶活性。
这些观察结果在证实白色念珠菌相对于热带念珠菌具有更高毒力的同时，首次证明了抗真菌药物对这些常见机会性病原体细胞外磷脂酶的影响。
