Shimada Shigeki, Iwabuchi Kazuya, Watano Keiko, Shimizu Hidemi, Yamada Hideto, Minakami Hisanori, Onoé Kazunori
Division of Immunobiology, Research Section of Pathophysiology, Institute for Genetic Medicine, Hokkaido University, Sapporo, Japan.
Am J Reprod Immunol. 2003 Jul;50(1):104-12. doi: 10.1034/j.1600-0897.2003.00060.x.
To investigate whether the allograft inflammatory factor-1 (AIF-1) is expressed and plays a role in the reproductive system.
AIF-1 expression was examined in uteri of non-pregnant and pregnant mice by Northern blot analysis, RT-PCR and immunohistochemistry.
The expression of AIF-1 varied during the estrous cycle with a peak at estrus. After the insemination, the expression of AIF-1 mRNA diminished gradually and again increased in the pre-implantation or implantation period in allogeneic or syngeneic pregnancy, respectively. Enhanced expressions of AIF-1, tumor necrosis factor-alpha (TNF-alpha) and nitric oxide synthase 2 (NOS2) mRNA were observed in the embryos of resorption-prone pregnancy injected with poly(I:C).
This study demonstrated for the first time that AIF-1 was expressed in uterus. The expression level was associated with the population size of macrophage and varied during the estrous cycle and the pregnancy period. The augmented expression of AIF-I with concomitant expressions of TNF-alpha and NOS2 mRNA in poly(I:C)-injected mice suggests a correlation between AIF-1 production and fetal resorption.
研究同种异体移植炎症因子-1（AIF-1）是否在生殖系统中表达并发挥作用。
通过Northern印迹分析、逆转录-聚合酶链反应（RT-PCR）和免疫组织化学检测未孕和孕鼠子宫中AIF-1的表达。
AIF-1的表达在发情周期中有所变化，在发情期达到峰值。受精后，AIF-1 mRNA的表达逐渐减少，在同种异体或同基因妊娠的植入前或植入期又再次增加。在注射聚肌苷酸胞嘧啶核苷酸（poly(I:C)）的易发生吸收的妊娠胚胎中，观察到AIF-1、肿瘤坏死因子-α（TNF-α）和一氧化氮合酶2（NOS2）mRNA的表达增强。
本研究首次证明AIF-1在子宫中表达。其表达水平与巨噬细胞数量相关，并在发情周期和妊娠期有所变化。在注射poly(I:C)的小鼠中，AIF-1表达增加并伴有TNF-α和NOS2 mRNA的表达，提示AIF-1产生与胎儿吸收之间存在关联。
