Vleghels Ingrid, Hontelez Jan, Ribeiro Ana, Fransz Paul, Bisseling Ton, Franssen Henk
Department of Molecular Biology, Wageningen University, Drijenlaan 3, 6703 HA Wageningen, The Netherlands.
Planta. 2003 Nov;218(1):42-9. doi: 10.1007/s00425-003-1081-9. Epub 2003 Sep 24.
In legumes, ENOD40 expression is increased upon interaction of plants with rhizobia. Little is known of the expression pattern of ENOD40 during other stages of the plant life cycle. Studies of ENOD40 expression in non-legume development may give an indication of the function of the gene. To investigate the ENOD40 expression pattern during plant development, a fusion between the beta-glucuronidase (GUS) reporter gene and 150 bp of the 5' untranslated region plus 3,000 bp of 5' untranscribed tomato ENOD40 sequence was constructed and introduced into Lycopersicon esculentum Miller. Based on the observed GUS expression patterns in transgenic tomato we speculate that ENOD40 in tomato has a role in counteracting ethylene-provoked responses.
在豆科植物中，植物与根瘤菌相互作用时，ENOD40的表达会增加。对于ENOD40在植物生命周期其他阶段的表达模式，人们了解甚少。对非豆科植物发育过程中ENOD40表达的研究可能会揭示该基因的功能。为了研究植物发育过程中ENOD40的表达模式，构建了β-葡萄糖醛酸酶（GUS）报告基因与150 bp的5'非翻译区加上3000 bp的5'未转录番茄ENOD40序列的融合体，并将其导入番茄（Lycopersicon esculentum Miller）。基于在转基因番茄中观察到的GUS表达模式，我们推测番茄中的ENOD40在对抗乙烯引发的反应中发挥作用。
