Parra M M, del Pozo O, Luna R, Godoy J A, Pintor-Toro J A
Instituto de Recursos Naturales y Agrobiología, C.S.I.C., Sevilla, Spain.
Plant Mol Biol. 1996 Nov;32(3):453-60. doi: 10.1007/BF00019097.
We have isolated a genomic clone encoding tomato TAS14, a dehydrin that accumulates in response to mannitol, NaCl or abscisic acid (ABA) treatment. A fragment of tas14 gene containing the region from -2591 to +162 fused to beta-glucuronidase gene drives ABA- and osmotic stress-induced GUS expression in transgenic tobacco. Histochemical analysis of salt-, mannitol- and ABA-treated plants showed GUS activity mainly localized to vascular tissues, outer cortex and adventitious root meristems, coinciding with the previously observed distribution of TAS14 protein in salt-stressed tomato plants. In addition, GUS activity was also observed in guard cells, trichomes and leaf axils. Developmentally regulated gus expression was studied in unstressed plants and found to occur not only in embryos, but also in flowers and pollen. Tas14 expression in floral organs was confirmed by northern blots of tomato flowers.
我们分离出了一个编码番茄TAS14的基因组克隆，TAS14是一种脱水蛋白，在甘露醇、NaCl或脱落酸（ABA）处理后会积累。tas14基因的一个片段，包含从-2591到+162的区域，与β-葡萄糖醛酸酶基因融合，驱动转基因烟草中ABA和渗透胁迫诱导的GUS表达。对盐、甘露醇和ABA处理的植物进行组织化学分析表明，GUS活性主要定位于维管组织、外皮层和不定根分生组织，这与之前在盐胁迫番茄植株中观察到的TAS14蛋白分布一致。此外，在保卫细胞、毛状体和叶腋中也观察到了GUS活性。在未受胁迫的植物中研究了发育调控的gus表达，发现其不仅在胚胎中发生，在花和花粉中也有表达。番茄花的Northern杂交证实了Tas14在花器官中的表达。
