Hanski C, Bornhoeft G, Shimoda T, Hanski M L, Lane D P, Stein H, Riecken E O
Department of Gastroenterology, Klinikum Steglitz, Freien Universität Berlin, Germany.
Cancer. 1992 Dec 15;70(12):2772-7. doi: 10.1002/1097-0142(19921215)70:12<2772::aid-cncr2820701208>3.0.co;2-5.
This study was performed to determine whether morphologic differences of colonic cancer types can be related to different genotypes of these tumors.
Paraffin sections of 76 human invasive colorectal carcinomas were examined for the overexpression of p53 oncoprotein with the avidin-biotin complex-peroxidase staining procedure and CM-1 antiserum, which detects p53 protein in paraffin-embedded material. The tumors were categorized as mucinous (22 cases), most of which originated from adenomas, and nonmucinous, which were subdivided into carcinomas originating from adenoma-carcinoma sequence (ACS) (29 cases) and de novo (DN) carcinomas (25 cases).
Nineteen DN carcinomas (76%), 21 ACS carcinomas (72%), and 8 mucinous carcinomas (36%) exhibited detectable amounts of p53 protein in the tumor cell nuclei. Strong overexpression of p53 protein coincided with a high percentage (> 40%) of stained nuclei in 40% of ACS and 48% of DN carcinomas versus 9% of mucinous tumors. The percentage of stained nuclei, intensity of staining, and distribution of the stained areas did not correlate with the grade of differentiation or the invasive edge of the tumors. Along with nuclear staining of the tumor area, a distinct perinuclear staining of normal epithelial cells adjacent to the tumor was observed in 48% of DN, 7% of ACS, and 9% of mucinous carcinomas.
The current results, in combination with the recently published data on Ki-ras and c-myc alterations, indicate that mucinous carcinomas differ from nonmucinous colorectal carcinomas in their genetic lesions.
本研究旨在确定结肠癌类型的形态学差异是否与这些肿瘤的不同基因型相关。
采用抗生物素蛋白-生物素复合物-过氧化物酶染色法及CM-1抗血清，检测76例人类浸润性结直肠癌石蜡切片中p53癌蛋白的过表达情况，该抗血清可检测石蜡包埋材料中的p53蛋白。肿瘤分为黏液性（22例），其中大部分起源于腺瘤，以及非黏液性，后者又细分为起源于腺瘤-癌序列（ACS）的癌（29例）和原发性（DN）癌（25例）。
19例DN癌（76%）、21例ACS癌（72%）和8例黏液性癌（36%）在肿瘤细胞核中显示可检测到的p53蛋白量。p53蛋白的强过表达与40%的ACS癌和48%的DN癌中高比例（>40%）的染色细胞核一致，而黏液性肿瘤为9%。染色细胞核的百分比、染色强度和染色区域的分布与肿瘤的分化程度或浸润边缘无关。除肿瘤区域的核染色外，在48%的DN癌、7%的ACS癌和9%的黏液性癌中观察到肿瘤相邻正常上皮细胞有明显的核周染色。
目前的结果与最近发表的关于Ki-ras和c-myc改变的数据相结合，表明黏液性癌在基因病变方面与非黏液性结直肠癌不同。
