Zhou Zhi, Zhou Rujun, Tang Zhen
Department of Preventive Dentistry, West China College of Stomatology, Sichuan University, Chengdu 610041, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2003 Aug;21(4):295-7.
To evaluate the inhibitory effects of topical application of specific immunoglobulin yolk antibodies (IgY) to mutans streptococci on composition of human dental plaque.
A total of 24 healthy adult volunteers were selected and randomly distributed into three experimental groups. After a supragingival scaling, the subjects were treated with an application of IgY containing spray (test group, n = 9), a 0.15% chlorhexidine(CH) spray(positive control group, n = 9) or a placebo phosphate buffered saline(PBS) spray without any active ingredients(negative control group, n = 6) three times daily for three weeks. Pooled plaque samples from the smooth surfaces of teeth were collected for microbiologic analysis before administration (baseline, day 0), during treatment(days 7, 14 and 21) and after withdrawal of the agents(days 28, 42 and 56) and plaque index were recorded. Microbial cultivation were performed by serially diluting the samples with PBS solution an cultivating the aliquots on mitis salivarius agar(MS) and MS supplemented with bacitracin (MSB) for oral streptococci and mutans streptococci respectively. The number of colony-forming unit (CFU) per plate was counted and the percentage of mutans streptococci per oral streptococci in dental plaque were determined. The effect of IgY on colonization of mutans streptococci were analyzed by an analysis of variance (ANOVA).
The plaque indexes remained unchanged, but mutans streptococci in dental plaque was suppressed significantly following a short term(three weeks) application of IgY, and low level of mutans streptococci persisted for at least 5 weeks after withdrawal of IgY.
The results suggest that the immunization with specific immunoglobulin yolk against mutans streptococci could be useful for long term suppression of mutans streptococci colonization in human dental plaque.
评估局部应用特异性抗变形链球菌免疫球蛋白蛋黄抗体（IgY）对人牙菌斑成分的抑制作用。
选取24名健康成年志愿者，随机分为三个实验组。龈上洁治后，受试者分别接受含IgY喷雾治疗（试验组，n = 9）、0.15%氯己定（CH）喷雾治疗（阳性对照组，n = 9）或不含任何活性成分的安慰剂磷酸盐缓冲盐水（PBS）喷雾治疗（阴性对照组，n = 6），每日三次，持续三周。在给药前（基线，第0天）、治疗期间（第7、14和21天）以及停药后（第28、42和56天）收集牙齿光滑表面的混合菌斑样本进行微生物分析，并记录菌斑指数。通过用PBS溶液对样本进行系列稀释，并分别在含杆菌肽的唾液链球菌琼脂（MS）和MSB培养基上培养等分试样，对口腔链球菌和变形链球菌进行微生物培养。计算每平板的菌落形成单位（CFU）数量，并测定牙菌斑中变形链球菌占口腔链球菌的百分比。采用方差分析（ANOVA）分析IgY对变形链球菌定植的影响。
菌斑指数保持不变，但短期（三周）应用IgY后，牙菌斑中的变形链球菌被显著抑制，停药后至少5周变形链球菌水平仍维持在较低水平。
结果表明，用特异性抗变形链球菌免疫球蛋白蛋黄进行免疫接种可能有助于长期抑制人牙菌斑中变形链球菌的定植。
