Shirasaka Kunitaka, Morikawa Hiroshi
Department of Otolaryngology and Bronchoesophagology, Dokkyo University School of Medicine, Tochigi.
Nihon Jibiinkoka Gakkai Kaiho. 2003 Aug;106(8):823-30. doi: 10.3950/jibiinkoka.106.823.
CD80 and CD86, which are costimulatory molecules in T-cell activation, play important roles in the differentiation of Th1- or Th2-phenotypes. The results of blocking studies using neutralizing antibodies have suggested that CD80 and CD86 also play important roles in sensitization to cedar pollen antigen, but very few studies have examined the kinetics of CD80 and CD86 expression on antigen-presenting cells (APC). We studied the kinetics of CD80 and CD86 expression on APC after allergen-stimulation in cedar pollinosis subjects. A skin-prick test was performed in nine subjects with pollinosis and seven control subjects. Peripheral blood mononuclear cells (PBMC) were isolated and stimulated with Japanese cedar pollen extract. Zero to 8 days following in vitro stimulation, the expression of CD80 and CD86 in either CD14+ or CD19+ cells was analyzed by two-color flow cytometry. The expression of CD28, CTLA-4 and CD40L on CD4+ cells was analyzed by two-color flow cytometry after eliminating either CD14+ or CD19+ cells. After in vitro stimulation, the expression of both CD80 and CD86 was significantly upregulated in pollinosis subjects compared to control subjects. However, the difference was observed in the kinetics of CD80 and CD86 expression following allergen stimulation. The expression of CD86 was upregulated earlier than that of CD80 after in vitro stimulation. In the absence of CD19+ cells, the expression of CD28, CTLA-4, and CD40L in CD4+ cells was significantly lower than that in the absence of CD14+ cells. These results indicate that CD19+ cells of pollinosis subjects expressed higher CD80 and CD86 than that of control subjects, and that the kinetics of CD80 and CD86 expression following stimulation differed. In pollinosis subjects, CD19+ cells may thus function as APC in allergen-induced activation of PBMC.
CD80和CD86是T细胞活化中的共刺激分子，在Th1或Th2表型的分化中起重要作用。使用中和抗体进行的阻断研究结果表明，CD80和CD86在对雪松花粉抗原的致敏中也起重要作用，但很少有研究检测抗原呈递细胞（APC）上CD80和CD86表达的动力学。我们研究了雪松花粉症患者变应原刺激后APC上CD80和CD86表达的动力学。对9名花粉症患者和7名对照受试者进行了皮肤点刺试验。分离外周血单个核细胞（PBMC）并用日本雪松花粉提取物刺激。体外刺激后0至8天，通过双色流式细胞术分析CD14 +或CD19 +细胞中CD80和CD86的表达。在去除CD14 +或CD19 +细胞后，通过双色流式细胞术分析CD4 +细胞上CD28、CTLA-4和CD40L的表达。体外刺激后，与对照受试者相比，花粉症患者中CD80和CD86的表达均显著上调。然而，在变应原刺激后CD80和CD86表达的动力学方面观察到差异。体外刺激后，CD86的表达比CD80上调得更早。在没有CD19 +细胞的情况下，CD4 +细胞中CD28、CTLA-4和CD40L的表达明显低于没有CD14 +细胞的情况。这些结果表明，花粉症患者的CD19 +细胞比对照受试者表达更高的CD80和CD86，并且刺激后CD80和CD86表达的动力学有所不同。因此，在花粉症患者中，CD19 +细胞可能在变应原诱导的PBMC活化中作为APC发挥作用。
