Shan Jun-jie, Tian Geng-yuan
State Key Laboratory of Bio-organic and Natural Product Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Science, Shanghai 200032, China.
Yao Xue Xue Bao. 2003 Jun;38(6):438-41.
To isolate a complex polysaccharide (AMP-B) from Atractylodes macrocephala Koidz and study its phtsico-chemical properties and hypoglycemic activity.
The root of Atractylodes macrocephala K. was extracted with water and precipitated with ethanol, dialyzed against water and freeze-dried to get the crude polysaccharides (AMP). A complex polysaccharide (AMP-B) was isolated and purified on DEAE-cellulose column. The model of diabetes rats was established with alloxan injection through the tail vein. Male rats were divided into 5 groups: the normal group, the control group, and three AMP-B-fed groups. Measuring the blood glucose, water and food consumption, thymus and pancreas index, and studying cut sections of pancreas tissues.
AMP-B is a complex-polysaccharide, elemental analysis of AMP-B shown C 32.84%, H 5.68%, and N 1.79%. The neutral polysaccharide content of AMP-B was 50.3%, uronic acid was 40.4%, and protein was 11.5%. Monosaccharide composition of AMP-B was determined by GC, AMP-B composed of Glc, Gal, Man, Ara and Rha in a molar ratio of 3.0:2.5:1.3:3.5:1.0. AMP-B was found to reduce blood glucose level in alloxan-diabetic rats markedly at doses of 50, 100 and 200 mg.kg-1 by ig, but no effect in normal rat. AMP-B was found to decrease the consumption of water and food, recover pancreas damage of diabetic rats obviously, inhibited the atrophy of thymus and pancreas of the diabetic rats induced by alloxan.
AMP-B showed significant hypoglycemic effect on the experimental hyperglycemias rats induced by alloxan.
从白术中分离出一种复合多糖（AMP-B），并研究其理化性质和降血糖活性。
白术根用水提取，乙醇沉淀，水透析，冷冻干燥得粗多糖（AMP）。在DEAE-纤维素柱上分离纯化得到复合多糖（AMP-B）。通过尾静脉注射四氧嘧啶建立糖尿病大鼠模型。雄性大鼠分为5组：正常组、对照组和3个AMP-B喂养组。测定血糖、水和食物消耗量、胸腺和胰腺指数，并研究胰腺组织切片。
AMP-B是一种复合多糖，AMP-B的元素分析显示碳含量为32.84%，氢含量为5.68%，氮含量为1.79%。AMP-B的中性多糖含量为50.3%，糖醛酸为40.4%，蛋白质为11.5%。通过气相色谱法测定AMP-B的单糖组成，AMP-B由葡萄糖、半乳糖、甘露糖、阿拉伯糖和鼠李糖组成，摩尔比为3.0:2.5:1.3:3.5:1.0。发现AMP-B以50、100和200mg·kg-1的剂量灌胃可显著降低四氧嘧啶糖尿病大鼠的血糖水平，但对正常大鼠无影响。发现AMP-B可减少水和食物的消耗，明显恢复糖尿病大鼠的胰腺损伤，抑制四氧嘧啶诱导的糖尿病大鼠胸腺和胰腺萎缩。
AMP-B对四氧嘧啶诱导的实验性高血糖大鼠具有显著的降血糖作用。
