BMC Complement Altern Med. 2013 Jul 16;13:176. doi: 10.1186/1472-6882-13-176.
Gamijinhae-tang (GJHT) has long been used in Korea to treat respiratory diseases. The therapeutic effect of GJHT is likely associated with its anti-inflammatory activity. However, the precise mechanisms underlying its effects are unknown. This study was conducted to evaluate the protective effects of GJHT in a porcine pancreatic elastase (PPE) and lipopolysaccharide(LPS) induced animal model of acute lung injury (ALI).
In this study, mice were intranasally exposed to PPE and LPS for 4 weeks to induce chronic obstructive pulmonary disease (COPD)-like lung inflammation. Two hours prior to PPE and LPS administration, the treatment group was administered GJHT extracts via an oral injection. The numbers of neutrophils, lymphocytes, macrophages and total cells in the bronchoalveolar lavage (BAL) fluid were counted, and pro-inflammatory cytokines were also measured. For histologic analysis, hematoxylin and eosin (H&E) stains and periodic acid-Schiff (PAS) stains were evaluated.
After inducing ALI by treating mice with PPE and LPS for 4 weeks, the numbers of neutrophils, lymphocytes and total cells were significantly lower in the GJHT group than in the ALI group. In addition, the IL-1β and IL-6 levels were significantly decreased in the GJHT group. The histological results also demonstrated the attenuation effect of GJHT on PPE- and LPS-induced lung inflammation.
The results of this study indicate that GJHT has significantly reduces PPE- and LPS-induced lung inflammation. The remarkable protective effects of GJHT suggest its therapeutic potential in COPD treatment.
甘桔冰梅片（GJHT）在韩国长期以来一直被用于治疗呼吸系统疾病。GJHT 的治疗效果可能与其抗炎活性有关。然而，其确切作用机制尚不清楚。本研究旨在评估 GJHT 在猪胰弹性蛋白酶（PPE）和脂多糖（LPS）诱导的急性肺损伤（ALI）动物模型中的保护作用。
在这项研究中，小鼠通过鼻腔暴露于 PPE 和 LPS 4 周，以诱导类似于慢性阻塞性肺疾病（COPD）的肺炎症。在给予 PPE 和 LPS 之前 2 小时，治疗组通过口服注射给予 GJHT 提取物。计数支气管肺泡灌洗液（BAL）中的中性粒细胞、淋巴细胞、巨噬细胞和总细胞数量，并测量促炎细胞因子。进行组织学分析，评估苏木精和伊红（H&E）染色和过碘酸希夫（PAS）染色。
通过用 PPE 和 LPS 处理小鼠 4 周诱导 ALI 后，GJHT 组的中性粒细胞、淋巴细胞和总细胞数量明显低于 ALI 组。此外，GJHT 组的 IL-1β 和 IL-6 水平显著降低。组织学结果还表明 GJHT 对 PPE 和 LPS 诱导的肺炎症具有减弱作用。
本研究结果表明，GJHT 可显著减轻 PPE 和 LPS 诱导的肺炎症。GJHT 的显著保护作用表明其在 COPD 治疗中的潜在治疗价值。
