Kim Han-Woo, Kim Young-Ok, Lee Jeong-Ho, Kim Kyung-Kil, Kim Yung-Jin
Biotechnology Research Center, National Fisheries Research and Development Institute, 408-1 Sirangri, Gigang-eup, Gigang-gun, Busan 619-902, Korea.
Biotechnol Lett. 2003 Aug;25(15):1231-4. doi: 10.1023/a:1025020309596.
Citrobacter braakii YH-15 produced an intracellular phytase which was purified 12800 fold to homogeneity with the specific activity of 3457 units mg(-1), which is 1.9 times higher than E. coli phytase previously recorded as having the highest specific activity. Its molecular weight was 47 kDa by SDS-PAGE gel. Enzyme activity was optimal at pH 4 and at 50 degrees C. The Km value for sodium phytate was 0.46 mM with a Vmax 6027 U mg(-1). The phytase was resistant to proteases such as trypsin, pepsin, papain, pancreatin, and elastase.
布氏柠檬酸杆菌YH-15产生一种胞内植酸酶，该酶经纯化12800倍后达到同质，比活性为3457单位mg(-1)，比先前记录的具有最高比活性的大肠杆菌植酸酶高1.9倍。通过SDS-PAGE凝胶测定其分子量为47 kDa。酶活性在pH 4和50℃时最佳。植酸钠的Km值为0.46 mM，Vmax为6027 U mg(-1)。该植酸酶对诸如胰蛋白酶、胃蛋白酶、木瓜蛋白酶、胰酶和弹性蛋白酶等蛋白酶具有抗性。
