Li Lan, Zhang Wei-yuan
Department of Pathology, Guiyang Medical College, Guiyang 550004, China.
Zhonghua Bing Li Xue Za Zhi. 2003 Aug;32(4):347-9.
To evaluate the roles of p27(kip1), p16 gene protein and proliferating cell nuclear antigen expression in nasopharyngeal carcinoma (NPC).
The EnVision immunohistochemical method was used to detect the expression of p27(kip1), p16 gene protein and PCNA in 66 cases of non-keratinized carcinoma (NKC) and 25 cases of non-tumor nasopharyngeal tissue.
(1) The positive expression rates of p27(kip1), p16 gene protein were 65%, 68% in NKC respectively. There were significant differences between NKC and non-tumor group (P < 0.05). (2) The expression of p27(kip1), p16 protein correlated with cranial nerve encroaching and the 5-year survival rates of the patients (P < 0.05), but had no significant correlation to lymph node metastases and clinical staging (P > 0.05). The expression of PCNA was related to clinical staging and to the patient's 5-year survival rates (P < 0.05), but not to lymph node metastases and cranial nerve encroaching (P > 0.05). (3) The positive expression of p27(kip1), p16 gene protein and PCNA were correlated.
The results suggest that immunological labeling of p27(kip1), p16 gene protein and PCNA might be used to determine the prognosis of NKC.
评估p27（kip1）、p16基因蛋白及增殖细胞核抗原表达在鼻咽癌（NPC）中的作用。
采用EnVision免疫组化法检测66例非角化癌（NKC）及25例鼻咽部非肿瘤组织中p27（kip1）、p16基因蛋白及PCNA的表达。
（1）NKC中p27（kip1）、p16基因蛋白的阳性表达率分别为65%、68%。NKC组与非肿瘤组之间差异有统计学意义（P<0.05）。（2）p27（kip1）、p16蛋白的表达与颅神经侵犯及患者的5年生存率相关（P<0.05），但与淋巴结转移及临床分期无显著相关性（P>0.05）。PCNA的表达与临床分期及患者的5年生存率相关（P<0.05），但与淋巴结转移及颅神经侵犯无关（P>0.05）。（3）p27（kip1）、p16基因蛋白及PCNA的阳性表达之间具有相关性。
结果提示，p27（kip1）、p16基因蛋白及PCNA的免疫标记可能用于判断NKC的预后。
