Shvedova Anna A, Castranova Vincent, Kisin Elena R, Schwegler-Berry Diane, Murray Ashley R, Gandelsman Vadim Z, Maynard Andrew, Baron Paul
Pathology and Physiology Research Branch, National Institute of Occupational Safety and Health, and Physiology and Pharmacology Department, West Virginia University, Morgantown 26505, USA.
J Toxicol Environ Health A. 2003 Oct 24;66(20):1909-26. doi: 10.1080/713853956.
Carbon nanotubes are new members of carbon allotropes similar to fullerenes and graphite. Because of their unique electrical, mechanical, and thermal properties, carbon nanotubes are important for novel applications in the electronics, aerospace, and computer industries. Exposure to graphite and carbon materials has been associated with increased incidence of skin diseases, such as carbon fiber dermatitis, hyperkeratosis, and naevi. We investigated adverse effects of single-wall carbon nanotubes (SWCNT) using a cell culture of immortalized human epidermal keratinocytes (HaCaT). After 18 h of exposure of HaCaT to SWCNT, oxidative stress and cellular toxicity were indicated by formation of free radicals, accumulation of peroxidative products, antioxidant depletion, and loss of cell viability. Exposure to SWCNT also resulted in ultrastructural and morphological changes in cultured skin cells. These data indicate that dermal exposure to unrefined SWCNT may lead to dermal toxicity due to accelerated oxidative stress in the skin of exposed workers.
碳纳米管是类似于富勒烯和石墨的碳同素异形体的新成员。由于其独特的电学、力学和热学性质，碳纳米管在电子、航空航天和计算机行业的新型应用中具有重要意义。接触石墨和碳材料与皮肤疾病发病率增加有关，如碳纤维皮炎、角化过度和痣。我们使用永生化人表皮角质形成细胞（HaCaT）的细胞培养物研究了单壁碳纳米管（SWCNT）的不良反应。在HaCaT暴露于SWCNT 18小时后，自由基的形成、过氧化产物的积累、抗氧化剂的消耗和细胞活力的丧失表明了氧化应激和细胞毒性。暴露于SWCNT还导致培养的皮肤细胞发生超微结构和形态变化。这些数据表明，皮肤接触未精制的SWCNT可能由于接触工人皮肤中氧化应激加速而导致皮肤毒性。
